Evaluation of Drug Resistance Before and After Biofilm Formation of Bacteria Causing Wound Infection and Detection of Their Protease Activity

Background: Wound infection is a highly common problem in hospital settings, where microbes are often resistant and difficult to treat due rapid exposure antibiotics. While treating wound infection, bacteria enter the deep tissue; as therapy needs long time, have sufficient time develop biofilm, which makes them much more Objectives: The current study was performed identify wound-infecting determine their protease production activity. Methods: ability produce biofilm evaluated by Congo red agar tube methods. Antibiotic resistance pattern assessed before after formation detect changes formation. Results: We identified Pseudomonas aeruginosa, Proteus mirabilis, vulgaris, Corynebacterium xerosis, Alcaligenes faecalis, Bacillus cereus, Escherichia coli, Acinetobacter spp., Klebsiella pneumoniae, Staphylococcus Shigella Salmonella spp. 20 samples, among about 10 isolates were found be producers. Almost all producers showed complete or smaller inhibition zone. Conclusions: Pathogenic can eradicate antibiotic treatment if they able biofilm; thus, it essential prevent